Journal: Neuromethods

Article Title: Single-Unit Extracellular Recording from the Cerebellum During Eyeblink Conditioning in Head-Fixed Mice

doi: 10.1007/978-1-4939-7549-5_3

Figure Lengend Snippet: Apparatus for head-fixed electrophysiology, optogenetics, and behavior experiments. The two photographs show a top view (left) and side view (right) of the assembled components. Aluminum foil electrical shields have been removed from equipment (e.g., infrared light (d)) for better visibility of materials. Labels reference components described in the materials section. Briefly: (a) Multi-Field Magnetic Speakers (TDT MF1). (b) BNC to LED cable (custom). (c) High-speed camera (AVT GE680). (d) Infrared light (Towallmark Crazy Cart 48-LED IR Infrared Night Vision Illuminator). (e) Blunt needle (23G), adapter (WPI 13160), and tubing for delivering air puff (Clippard URH1–0402-CLT). (f) Bars for holding headplate (custom). (g) Three-axis micromanipulator (Narishige UMC3). (h) Handheld microscope camera (Dino-Lite AD-4013 MTL). (i) One-axis micromanipulator (Narishige MMO-220A modified to remove ball joint). (j) Stereotaxic micromanipulator (Narishige SMM-100 with custom shaft (EDMS16–054b) and modified MA-2 micromanipulator stand (SDMS16–033a)). (k) Treadmill (custom). (l) Rotary encoder (Karlsson Robotics E6C2-CWZ3E 1024 count/revolution quadrature encoder)

Article Snippet: Materials Apparatus list-behavior=unordered prefix-word= mark-type=disc max-label-size=0 Stereotaxic micromanipulator (e.g., Narishige SMM-100 with custom shaft (EDMS16–054b) and modified MA-2 micromanipulator stand (SDMS16–033a)) ( ) One-axis micromanipulator (e.g., Narishige MMO-220A one-axis oil hydraulic micromanipulator modified to remove the ball joint) ( ) Handheld microscope camera (Dino-Lite AD-4013 MTL) ( ) Adapter for microscope camera (Dino-Lite HD-P1) Three-axis manipulator for microscope camera (e.g. Narishige UMC3) ( ) 10 mm OD post (if you use the UMC3) 7 mm OD post (for holding the camera adapter in the micromanipulator) Four 1⁄2” diameter 12” posts (Thorlabs) Two 1⁄2” diameter 6” posts (Thorlabs) Three right-angle mounts for 1⁄2” posts (Thorlabs RA90) 1/stimulus, third hand (e.g. alligator clip and stem from SUNYEE Helping Hand MZ101B) 1/stimulus, right-angle mounts that can accommodate the third hands (OptoSigma CCHN-12.7–6 is compatible with SUNYEE Helping Hand) Solid core copper ground wires and panel Supplies list-behavior=unordered prefix-word= mark-type=disc max-label-size=0 Custom dura scraper (see , ) Sterile saline Small cotton balls 7.5% povidone-iodine Electrodes (see Note 6 ) Optrodes (see below for construction instructions) Implantable optical fibers (e.g., Thorlabs or custom-made) Construction of the Recording Apparatus list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Attach your materials to the vibration isolation table in appropriate positions relative to the treadmill using the Thorlabs posts and RA90 right-angle connectors.

Techniques: Optogenetics, Microscopy, Modification